Full-length cDNA and Genome Medicine

Assessment of Redundancy and Full-Length Rate of Full-Length Enriched cDNA Libraries

Collection of full-length genes requires libraries with full-length cDNA insert, large-scale sequencing, library assessment, and high-speed sequence clustering. Here we focus on computational methods, such as newly developed computer programs, since our experimental methods had been published previously. Our purpose is the collection of full-length cDNAs, therefore the proportion of full-length...

An Integrated Database System for Full-Length Human cDNA in NEDO Full-Length Human cDNA Sequencing Project

Whole genome sequence comparisons and "full-length" cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation.

To evaluate the existing annotation of the Arabidopsis genome further, we generated a collection of evolutionary conserved regions (ecores) between Arabidopsis and rice. The ecore analysis provides evidence that the gene catalog of Arabidopsis is not yet complete, and that a number of these annotations require re-examination. To improve the Arabidopsis genome annotation further, we used a novel...

An integrated analysis and database system for full-length cDNA.

Annotation and database system of full-length cDNA sequences was developed. As the components of the system, ORF annotation system, functional annotation system based on database search results, mapping annotation system, and integrated retrieval and display system were developed. In the ORF annotation system integrated analyses using conventional tools are performed and useful retrieval interf...

Genome Wide Analysis of the Alternative Splicing Based on Full-Length cDNA Data

One of the striking features in metazoan genomes is that the alternative pre-mRNA splicing is observed in many genes [1] Is was assumed that for a long time that alternative splicing was an exceptional event and, in most cases, a unique combination for the assembly of exons occurs and the sequences of exons were uniquely spliced to an ORF. However, the completion of large genomic sequencing pro...